Lab Vision Corporation, a part of Thermo Fisher Scientific

PT Module^TM Deparaffinization and Heat-Induced Epitope Retrieval Solution (100X)

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Description: This product is designed to deparaffinize and perform Heat Induced Antigen Retrieval (HIER) on formalin fixed paraffin-embedded tissue sections mounted on glass microscope slides. Deparffinization is accomplished by melting the paraffin at the high temperature used in HIER (over 90degrees C) along with emulsification of the paraffin by detergent. Formaldehyde fixation impairs or totally destroys the immunoreactivity of many antigens and epitopes. The negative effect of formaldehyde fixation can be reversed successfully with enzymatic digestion for some markers while not for others. Non-enzymatic epitope unmasking techniques have been recently introduced to improve the immunoreactivity of many antigens in formaldehyde fixed tissues. Heat-Induced Epitope Retrieval (HIER) in EDTA buffer has been found to improve the reactivity of many antibodies in formal-fixed tissues. The product is designed to be used in the Lab Vision Pretreatment Module ^TM. Determination of use conditions in any other insrument is the responsibility of the user. The Lab Vision Pretreatment Module^TM is designed to be used with slide racks used in the Lab vision 360, 480, 720 Autostainer, Biocare Nemesis 7200 autostainer and the DAKOCytomation autostainer and AutostainerPlus. Use of any other slide holding technology is the responsibility of the user.

Storage: Store at room temperature. Each component is stable for 18 months.This buffer contains no preservatives. Store product at 2-8degrees centigrade for storage longer than 3 months.

Supplied As: Buffer solution as various pH levels. This is 100X stock solution and should be diluted 100-fold with distilled water befor use. Depending on pH of water, the pH of the solution may requier pH adjustment. Use the proper pH solution for the antibody being detected. The proper solution to use for a given antibody must be determined by the user.

Suggested Working Dilution: 1:100 with distilled water.

Suggested Test Size: 1.5L of solution is used in each PTM tank.

Please select from the items below to add to your shopping cart:

	Cat.#:TA-125-PM1X----125 ml (Citrate buffer,pH 6, 100X)
	Cat.#:TA-125-PM2X----125 ml (EDTA buffer,pH 8, 100X)
	Cat.#:TA-125-PM3X----125 ml (Tris-HCL buffer,pH 10, 100X)
	Cat.#:TA-125-PM4X----125 ml (EDTA buffer,pH 9, 100X)
	Cat.#:TA-250-PM1X----250 ml (Citrate buffer,pH 6, 100X)
	Cat.#:TA-250-PM2X----250 ml (EDTA buffer,pH 8, 100X)
	Cat.#:TA-250-PM3X----250 ml (Tris-HCL buffer,pH 10, 100X)
	Cat.#:TA-250-PM4X----250 ml (EDTA buffer,pH 9, 100X)