MultiVision Polymer Detection System/ Anti-Mouse-AP+Anti-Rabbit-HRP
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Description: MultiVision Polymer Detection System is a simple and robust one-step polymer detection system for visualization of two antigens simultaneously in blue and red. A primary antibody cocktail of choice may be used in conjunction with ready-to-use MultiVision polymer cocktail, composed of anti-mouse/alkaline phosphatase (AP) + anti-rabbit/horseradish peroxidase (HRP). This cocktail is specially formulated from MultiVision polymers that provide increased sensitivity, time-savings, and detection simplicity. The MultiVision AP and HRP polymers are innovative, patented technologies consisting of smaller amino acid polymer subunits that minimize conflicts in binding the target protein. The smaller polymer allows for decreased binding conflicts resulting in more consistent staining and better signal amplification. Ultimately, this gives the user higher sensitivity and antibody efficiency as well as better signal-to-noise ratios. This system is biotin-free, eliminating background staining.
Antigen Specificity: Anti-Mouse IgG (H+L) and Anti-Rabbit IgG (H+L)
Enzyme: Peroxidase and Phosphatase
Chromogen/Substrate: LVBlue/ LVRed
Precautions: Clinical cases should be evaluated within the context of the performance of appropriate controls. Inclusion of a negative control fixed and processed in the same manner as the patient specimen placed on every slide run in addition to the case tissue is strongly recommended. For the test to be considered valid, the negative control should be clean. In some instances, very faint staining may be observed and is deemed acceptable. In addition, it is recommended that a negative tissue control slide be included for every batch of samples processed and run on the Lab Vision Autostainer. This negative tissue control should be included to ensure that the other treatment procedures did not create false positive staining.
Supplied As: Ready-to-use polymer cocktail composed of MultiVision anti-rabbit/HRP + anti-mouse/AP polymers.
LVBlue for Alkaline Phosphatase activity and LVRed for Horseradish Peroxidase activity.
Storage and Stability: All components are stable for up to 12 months when stored at 2-8°C. Store chromogens away from light when possible.
Methodology: This MultiVision Polymer Detection System may be employed with any primary antibody cocktail to visualize the presence of two proteins simultaneously. After the specific antibodies are bound to their respective antigens in tissue sections, the rabbit and mouse primary antibodies are located by a specially formulated cocktail of secondary antibody polymers: MultiVision anti-rabbit/HRP + MultiVision anti-mouse/AP. In general, the aminuteso acid polymer is conjugated to AP or HRP enzymes and the Fab fragments of goat anti-rabbit or goat anti-mouse immunoglobulins. The polymer complex is then visualized with first the LVBlue chromogen for AP activity and after washing with the LVRed chromogen for HRP activity. A weak hematoxilin nuclear counterstain is not recommended.
