Diluting Procedures: To make 1X PBS solution dilute conctentrate 25X with distilled water. Use 40ml PBS concentrateper 960ml DI water. Measure and pour appropriate volume of 25X PBS concentrate into a mixing flask and add DI water to final volume. Stir briefly. The 1X solution should be pH 7.6+-0.2 The pH of the soution may require slight adjustment. Adjust pH with 1N HCL(if pH is high) or 1N NaOH (if pH is low).

Storage: Store Phosphate Buffered Saline at room temperature. Concentrate is stable for 24 months from date of manufacture. Diluted solution should be used within one week. if solution becomes cloudey, discard and make fresh solution. Note: The salts in the 25X solution are highly concentrated. Temperatures below 44 degrees F (6 degrees C) may cause salts to precipitate. If this occus, stir the solution at RT for about 30 mins to redissolve the salts (use an alcohol cleaned stir bar to prevent bacterial contamination.