Click here to view datasheet

Multivision

Description: Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes and ependymal cells of the central nervous system. MAb to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. The Ki67 index or proliferation index is also valuable in predicting patient outcome. The use of the GFAP-Ki67 antibody pair has predictive value in astrocytoma cases. The use of rabbit monoclonal Ki67 allows for superior staining, in both sensitivity and specificity.For detection of the primary antibodies a ready-to-use MultiVision polymer cocktail consisting of anti-mouse/ horseradish peroxidase (HRP) + anti-rabbit/ alkaline phosphatase (AP) (Cat.# TL-012-MHRA) is available. This cocktail is specially formulated from MultiVision polymers that provide increased sensitivity, time-savings, and detection simplicity. The MultiVision AP and HRP polymers are innovative, patented technology. The smaller amino acid polymer subunits minimize conflicts in binding the target protein resulting in more consistent staining and better signal amplification. Ultimately, this gives the user higher sensitivity and antibody efficiency as well as better signal-to-noise ratios. This system is biotin-free, which prevents background staining found with traditional biotin-based methods. The MultiVision polymer detection systems yield high quality double staining in less than 2 hours on formalin-fixed and paraffin-embedded tissue sections. The kits allow observation of two single-stained cells in red and blue and/or co-localization marked by a purple/brownish mixed-color.

Epitope: GFAP – Not determined; Ki67- C-terminus

Clone Designation: GFAP – GA-5; Ki67- SP6

Immunogen: GFAP - Porcine spinal cord; Ki67– A synthetic peptide derived from the human Ki-67 protein

Positive Control:Some high grade astrocytomas

Molecular Weight:GFAP –51-52KDa; Ki67- unknown;

Species reactivity:Human. Others-not known

Cellular Localization:GFAP – Cytoplasmic; Ki67 – Nuclear