Adenovirus Fiber (Monomer and Trimer) Ab-4

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Compare different antibodies against Adenovirus.

Mouse Monoclonal Antibody

Description: Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends, and a knob at the C-terminus that contains the type-specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the viron.

Comments: Ab-4 recognizes both monomers and trimers, as judged by reactivity with both boiled (monomers (62kDa) only) and unboiled (both monomers and trimers (180-200kDa bands)) fiber on Western blots and by indirect immunofluorescence studies of cells infected with a temperature-sensitive Ad5 fiber mutant (H5ts142) which does not form trimers at the nonpermissive temperature at both 32C and 39.5C.

IgIsotype: IgG2a / kappa

Epitope: N-terminus, probably the first 17aa.

Clone Designation: 4D2

Immunogen: UV-irradiated Ad2 virus.

Positive Control:Adenovirus infected cells and tissues.

Cellular Localization:Nuclear

Molecular Weight:62kDa (monomer) and 180-200kDa (trimer)

Species reactivity:Adenovirus.